How to separate phospholipids with HPLC - Tips & Suggestions
January 4, 2013
/
/
/
/
/
/
/
/
/
/
/
/
/
/
/
Phospholipids present a challenge for chromatographers for various reasons.
Here some points to consider with suggested solutions.
Here some points to consider with suggested solutions.
- They have negligible UV absorption and generally require other detection methods.
- Subtle structural differences among phospholipids often lead to poor selectivity or co-elution.
- For detection, refractive index (RI), charged aerosol detection (CAD), ELSD (Evaporative Light Scattering Detection), or other "universal detectors" are recommended.
- As for the separation, Organic Normal Phase (ONP) using non polar solvents and a Cogent Silica-C™ column has been observed to produce excellent resolution.