How can I separate methacrylic acid and dodecyl benzene sulfonic acid - FAQ
December 17, 2012
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Good retention and peak shapes have been observed for similar compounds.
Methacrylic acid is similar in structure to acrylic acid, which can be well-retained with a mobile phase of DI water + 0.1% formic acid and a Bidentate C18 column.
Dodecyl benzene sulfonic acid has some of the same functional groups as docusate sodium, which also has a sulfonate group. The latter was observed to elute with a good peak shape using a gradient method and the Cogent Bidentate C18™ column. Dodecyl benzene sulfonic acid by itself should be suitable for the Cogent Diamond Hydride column as well, using an ammonium acetate-based mobile phase.
A gradient starting at high water (to retain the methacrylic acid) and ending at significant organic (to elute the dodecyl benzene sulfonic acid) can be used to retain both compounds. Since a Cogent Bidentate C18™ column was used for the two compounds above, it should be suitable for a separation of methacrylic acid and dodecyl benzene sulfonic acid as well. The Cogent Phenyl Hydride™ column would also be suitable.
Cogent Bidentate C18 ordering information
Cogent Diamond Hydride ordering information
Cogent Phenyl Hydride ordering information
Methacrylic acid is similar in structure to acrylic acid, which can be well-retained with a mobile phase of DI water + 0.1% formic acid and a Bidentate C18 column.
Dodecyl benzene sulfonic acid has some of the same functional groups as docusate sodium, which also has a sulfonate group. The latter was observed to elute with a good peak shape using a gradient method and the Cogent Bidentate C18™ column. Dodecyl benzene sulfonic acid by itself should be suitable for the Cogent Diamond Hydride column as well, using an ammonium acetate-based mobile phase.
A gradient starting at high water (to retain the methacrylic acid) and ending at significant organic (to elute the dodecyl benzene sulfonic acid) can be used to retain both compounds. Since a Cogent Bidentate C18™ column was used for the two compounds above, it should be suitable for a separation of methacrylic acid and dodecyl benzene sulfonic acid as well. The Cogent Phenyl Hydride™ column would also be suitable.
Cogent Bidentate C18 ordering information
Cogent Diamond Hydride ordering information
Cogent Phenyl Hydride ordering information