Reasons for using direct adaptive Di-Ad HPLC column connectors - Tips & Suggestions
February 28, 2022
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How to reduce peak broadening due to poor column connections
The spring-loaded "Direct Adaptive" Di-Ad™ HPLC column connectors were designed for speed and convenience when changing between different column brands on your instrument.In the example below, we demonstrate how this error-free process using DiAd fittings can also reduce peak broadening from common poor connections.
| Trace | Fitting Used | Height (mAU) | Plates (N) |
| Black | PEEK | 31.33 | 6306 |
| Red | Poorly Inserted PEEK | 30.36 | 6023 |
| Blue | Di-Ad™ | 35.37 | 7160 |
Method Conditions:
Direct Adaptive HPLC column connector: Double end fitting
Catalog No.: 49910-10-DD
Dimensions: 0.010" ID x 1/16th" OD, 100mm long
Column: Cogent Bidentate C8™, 4μm, 100Å.
Catalog No.: 40008-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 40% acetonitrile / 60% DI water 0.1 % formic acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute
Detection: UV 254nm
Sample Preparation: 0.1mg / mL phenol in mobile phase.
Notes: Column efficiency: theoretical plates (N) 1/2 Height N = 5.54((tR/W)2)
tR = Retention time of peak
W = Width of peak measured at 1/2 height
