Why are ionized polar compounds generally better retained in Aqueous Normal Phase ANP HPLC - FAQ
April 14, 2020
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The highest polarity is generally found in ionized compounds due to the fixed charge on the compound.
Therefore in Aqueous Normal Phase ANP HPLC, strongest retention is attained with the highest polarity, when the compounds are fully ionized.
Therefore in Aqueous Normal Phase ANP HPLC, strongest retention is attained with the highest polarity, when the compounds are fully ionized.