Choosing the Right Vial for Low-Volume Protein Studies
When working with low-volume protein samples, vial selection plays a critical role in preserving sample integrity and ensuring accurate results. Proteins can adsorb to borosilicate glass surfaces in two primary ways:
- Through hydroxyl groups or silanols present on untreated glass surfaces
- Via hydrophobic interactions with the glass itself
These interactions can lead to sample loss, inconsistent concentrations, and compromised data quality.
Recommended Solution: RSA-Pro X™ Vials
Deactivated RSA-Pro X™ vials are specifically designed to address these challenges. They offer:
- Advanced surface treatment that is more complete than any other glass vial available
- Non-stick, hydrophobic surface that resists protein binding
- Hydrolytic stability, even under thermal stress from heating and cooling cycles
This makes RSA-Pro X™ vials the optimal choice for sensitive, low-volume protein applications where sample recovery and consistency are essential.

RSA -Pro X vial product page and demonstration video
Alternately, if you can use plastic vials, your best option is most likely using the 300ul LCMS Poly Vials or our 700ul LCMS Poly Vials.

