How to keep surface treated frits from biofouling - FAQ
September 11, 2021
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Use non surfactant, Brij-35 to reduce biofouling
When many proteins get trapped on or adhere to stainless steel frits that are hydrophobic like our metal free surfaces, it is a good idea to add Brig-35 to a wash buffer to facilitate removal of very sticky proteins including BSA, mouse IgG and human plasma proteins.
Brig-35 will not diminish or change the effectiveness of the metal free surface. It is important to work in low concentrations of any ionic polymer to minimize biofouling due to surfaces.
When many proteins get trapped on or adhere to stainless steel frits that are hydrophobic like our metal free surfaces, it is a good idea to add Brig-35 to a wash buffer to facilitate removal of very sticky proteins including BSA, mouse IgG and human plasma proteins.
Brig-35 will not diminish or change the effectiveness of the metal free surface. It is important to work in low concentrations of any ionic polymer to minimize biofouling due to surfaces.
