How can I fix poor peak shape for phosphorylated compounds in aqueous normal phase ANP methods - FAQ
April 14, 2020
/
/
/
/
/
We have a number of strategies for fixing problems with peak shape for phosphorylated compounds:
  • Ensure the column is not overloaded. We recommend an injection volume of 1 µL or less with aqueous normal pase ANP methods.
  • Use Teflon bottles instead of borosilicate glass for your mobile phase reservoirs. Sodium from the glass can cause peak shape problems for anionic compounds.
  • Use 5–10 microM EDTA or medronic acid in the mobile phase and sample diluent. This will help chelate metals that cause problems for phosphates.
  • Try adding 0.01% ammonia to the sample diluent.
  • Try using a Cogent, metal free, coated stainless steel column.

Related Articles

  1. Add 10 micro molar EDTA or medronic acid to mobile phase for better peak shapes - Tips & Suggestions
  2. Injection volume suggestions for Cogent TYPE-C HPLC columns - Tips & Suggestions
  3. When and why use Teflon bottles for your mobile phase reservoirs - FAQ

© Copyright 2025. MICROSOLV. All Rights Reserved. Website & Hosting by BlueTone Media