Cogent Diamond Hydride 2.o HPLC column retention time precision - AppNote
November 13, 2015
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This graph is from a study investigating the day to day retention time precision with two test solutes on the Cogent Diamond Hydride 2.o™ HPLC column
Mobile Phase:
--A: DI Water / 0.1% Formic Acid
--B: Acetonitrile / 0.1% Formic Acid
Flow Rate: 0.3mL / minute
Injection Volume: 0.2uL
Detection: UV 254nm
Gradient:
Cogent Diamond Hydride HPLC Column Ordering Information
Peaks:
Blue Trace: Phenylalanine
Red Trace: Phenylglycine
Mobile Phase:
--A: DI Water / 0.1% Formic Acid
--B: Acetonitrile / 0.1% Formic Acid
Flow Rate: 0.3mL / minute
Injection Volume: 0.2uL
Detection: UV 254nm
Gradient:
| Time (minutes) | %B |
| 0 | 90 |
| 6 | 20 |
| 7 | 20 |
| 8 | 90 |
Cogent Diamond Hydride HPLC Column Ordering Information