If I use a buffer at pH 9 at 30 to 35C with the Zero Flow capillaries what lifetime should I expect from them - FAQ
December 20, 2012
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If you use a pH buffer even with moderate ionic strength, at pH 9, the capillaries would not be expected to last very long.
It is hard to predict how many runs you get because it will depend on your run times, your conditioning step, etc. However, these capillaries are not designed for a pH higher than 8. The silica was and the organo-silane bond of the polyacrylamide is susceptible to hydrolysis, degradation and pitting at high ionic strength buffers above pH 8.
Click HERE for capillaries for CE ordering information and pictures
It is hard to predict how many runs you get because it will depend on your run times, your conditioning step, etc. However, these capillaries are not designed for a pH higher than 8. The silica was and the organo-silane bond of the polyacrylamide is susceptible to hydrolysis, degradation and pitting at high ionic strength buffers above pH 8.
Click HERE for capillaries for CE ordering information and pictures