Can the L3 Linearity Solution in the HSQ kit be used for UV wavelength accuracy - FAQ
October 15, 2020
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The short answer is yes.
Many other qualification vendors use only caffeine for wavelength accuracy, at 205nm and 273nm. Holmium oxide of course covers 241-641nm, and is directly traceable to NIST standards. However, if you want, there is no reason you could not simply take the spectrum of caffeine as well.
The L3 Solution provides a nice, mid-range absorbance value, compatible with most UV-Vis detectors. You could of course use any other concentration that gives a suitable response. The reference solution would be mobile phase, but there is very little difference if wanted wanted to use water.
Many other qualification vendors use only caffeine for wavelength accuracy, at 205nm and 273nm. Holmium oxide of course covers 241-641nm, and is directly traceable to NIST standards. However, if you want, there is no reason you could not simply take the spectrum of caffeine as well.
The L3 Solution provides a nice, mid-range absorbance value, compatible with most UV-Vis detectors. You could of course use any other concentration that gives a suitable response. The reference solution would be mobile phase, but there is very little difference if wanted wanted to use water.