What is the elution order for analytes in the Resolution Test Mixture supplied in the HSQ kit - Tech Information
July 7, 2012
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The peaks / analytes elute in the following order for the PQ Kit™:
  1. Uracil
  2. Theophylline
  3. Caffeine
  4. 8-Chloro-Theophylline
For more recent and more efficient HPLC’s, the HSQ Kit™ has a different Resolution Test Mixture with one additional, earlier eluting peak. This additional peak allows the HSQ software to best determine your system’s extra column volume. This extra peak is not required but may be preferred.
  1. Uracil
  2. Theobromine
  3. Theophylline
  4. Caffeine
  5. 8-Chloro-Theophylline

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