Injection volume suggestions for Cogent TYPE-C HPLC columns - Tips & Suggestions
April 2, 2012
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When using an analytical Cogent TYPE-C™ column it is recommended to inject 1uL to 10uL of the sample which is prepared either in a diluent that matches, as closely as possible, the starting mobile phase composition of a gradient method or the method's isocratic mobile phase.
Larger volume injections that are performed on the columns are probably overloading the columns.
Larger volume injections that are performed on the columns are probably overloading the columns.