Molnupiravir Analyzed with an Inverse Gradient in HPLC - AppNote
October 12, 2021
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Oral Anti-Viral Medication - Aqueous Normal Phase Method
Molnupiravir can be analyzed using this simple "inverse gradient" HPLC Method shown below. This is offered as an orthogonal approach for a Reversed Phased Method and will show various polar impurities not detected by Reversed Phase HPLC.
Column: Cogent Diamond Hydride™, 4µm, 100Å
Dimensions: 4.6 x 75mm
Mobile Phase: (95:5) Acetonitrile / DI Water with 0.1% Formic Acid
Injection Volume: 1µL
Flow Rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Molnupiravir is dissolved at a concentration of 0.5mg / mL in (50:50) DI Water / Acetonitrile
Note: Molnupiravir is an oral antiviral drug that was developed for the treatment of influenza. It is a prodrug of the synthetic nucleoside derivative N4-hydroxycytidine, and exerts its antiviral action through introduction of copying errors during viral RNA replication.
Molnupiravir can be analyzed using this simple "inverse gradient" HPLC Method shown below. This is offered as an orthogonal approach for a Reversed Phased Method and will show various polar impurities not detected by Reversed Phase HPLC.
Peak:
Molnupiravir
Column: Cogent Diamond Hydride™, 4µm, 100Å
Dimensions: 4.6 x 75mm
Mobile Phase: (95:5) Acetonitrile / DI Water with 0.1% Formic Acid
Injection Volume: 1µL
Flow Rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Molnupiravir is dissolved at a concentration of 0.5mg / mL in (50:50) DI Water / Acetonitrile
Note: Molnupiravir is an oral antiviral drug that was developed for the treatment of influenza. It is a prodrug of the synthetic nucleoside derivative N4-hydroxycytidine, and exerts its antiviral action through introduction of copying errors during viral RNA replication.