Low carbon load · 100Å and 300Å pore sizes · Analytical and preparative formats · USP L26 equivalent
The Cogent RP C4™ HPLC Column by MICROSOLV, is a low‑carbon‑load reversed‑phase stationary phase designed for bioanalytical separations and moderately hydrophobic analytes. With reduced hydrophobicity compared to C8 and C18 phases, C4 columns provide controlled retention and excellent recovery for sensitive biomolecules.
These columns are commonly selected for the separation of proteins, peptides, enzymes, and other biologically relevant compounds, particularly where excessive retention or denaturation on more hydrophobic phases is a concern.
Designed for Bioanalysis and Mild Reversed Phase Retention
Cogent RP C4 columns utilize a short alkyl chain (C4) bonded to high‑purity silica, resulting in lower hydrophobic interaction strength. This makes them ideal for applications requiring gentler reversed‑phase conditions and improved analyte recovery.
Key characteristics include:
Low carbon loading for reduced hydrophobic interactions
Controlled retention for moderately hydrophobic and biological analytes
Excellent reproducibility for routine and validated methods
While primarily used in reversed‑phase HPLC, these columns may also be employed in normal‑phase methods when appropriate.
Particle Size and Pore Size Options
Cogent RP C4 columns are available in multiple porosity and particle size combinations to support a wide range of applications:
100Å pore size Ideal for small molecules and mildly hydrophobic compounds in standard reversed‑phase methods.
300Å pore size (most popular) Commonly used for small‑ to medium‑size proteins, peptides, and enzymes, providing improved mass transfer and resolution for larger biomolecules.
Available particle sizes:
3 µm
5 µm
10 µm
Both analytical and preparative column formats are offered.
Typical Applications
Cogent RP C4 columns are routinely used for:
Protein and peptide separations
Bioanalytical and biomolecule assays
Enzyme analysis
Applications requiring lower hydrophobic retention than C8 or C18
USP L26‑type methods and equivalent separations
Bio‑Compatible and Metal‑Free Column Options
For sensitive or metal‑interacting compounds, metal‑free column configurations are available. These columns feature stainless steel hardware with specialized surface treatments to minimize analyte interaction with metal surfaces.
Metal‑free columns are recommended when:
Analytes exhibit adsorption to stainless steel
Maximum bio‑compatibility is required
Improved recovery and reproducibility are critical
For availability and configuration details, contact MICROSOLV Customer Service or Technical Support.
Related Resources
View RP HPLC Column Specifications
Reference Information for Cogent RP™ HPLC Columns
Recommended Column Protection
To extend column lifetime and maintain performance, the MICROSOLV technical support team strongly recommends using a Cogent column filter with all HPLC columns whenever possible. A column filter is included with each column and helps protect against particulate contamination and sample matrix buildup.
Why Choose Cogent RP C4 Columns
Designed specifically for bioanalytical separations
Low hydrophobicity for improved recovery of biomolecules
Available in multiple pore sizes and particle sizes
Compatible with analytical and preparative workflows
Supported by MICROSOLV technical expertise Contact Customer Service using our Online Support form or call for details.
