Catalog Number - 68304-05P-4-3M

Cogent RP C4 Butyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of proteins, peptides, enzymes, and other large biomolecules. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger molecules compared to standard 100 Å pore columns. The result is improved mass transfer, excellent recovery, and reliable chromatographic performance for bioanalytical and biopharmaceutical applications.

68304-05P-4-3M is an HPLC column packed with 3 µm RP C4 Butyl™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 50 mm format. The short 50 mm length is optimized for rapid bioanalytical separations and increased sample throughput, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition and instrument configuration.

This column is ideal for proteins, peptides, enzymes, and other large hydrophobic or neutral compounds analyzed by reversed-phase HPLC. The wide pore structure allows biomolecules to fully access the bonded phase, improving retention and separation efficiency. Compared to C8 phases, the C4 chemistry generally provides lower retention, making it particularly useful for biomolecules that may be excessively retained on more hydrophobic stationary phases.

Key Benefits

• RP C4 Butyl™ Wide Pore stationary phase

• Fully double end capped

• Traditional butyl (C4) bonded phase

• Ultra-high purity silica support

• 300 Å wide pore design

• Optimized for proteins and peptides

• 3 µm particle technology

• Greater efficiency than 5 µm columns

• Fast analysis times

• Excellent for bioanalytical methods

Typical Applications

• Protein analysis

• Peptide analysis

• Enzyme characterization

• Bioanalytical methods

• Biopharmaceutical analysis

• Protein purity testing

• Peptide mapping

• Biomolecule separations

• Method development

• Reversed-phase HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 300 Å

• Column Dimensions: 4.0 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Special Order Item Information

• This is a Special Order Item.

• Learn more about Special Order Items and ordering policies.

• Contact Customer Service for quotation, availability, lead times, and ordering assistance.

Important Notes

• Designed for proteins, peptides, enzymes, and other large biomolecules

• 300 Å wide pores improve access to the bonded phase for larger molecules

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The short 50 mm length is ideal for rapid bioanalytical workflows

• Lower retention than comparable C8 stationary phases

• Excellent choice for biopharmaceutical and biotechnology laboratories

• This is a Special Order Item

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