HPLC Column, RP C4 Butyl, Wide Pore, 5um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for the separation of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides improved accessibility for large biomolecules while maintaining the reduced hydrophobic retention characteristic of C4 chemistry. The wide pore design makes it particularly well suited for bioanalytical applications where larger molecules require enhanced access to the bonded phase.
68504-05P-4-3M is an HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 50 mm format. The short 50 mm length is optimized for rapid analyses and high-throughput workflows, while the 5 µm particles provide low backpressure and robust operation. The 300 Å pore structure improves mass transfer and accessibility for larger biomolecules, making this column especially useful for bioanalytical separations. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.
This column is particularly useful for proteins, peptides, enzymes, and other large biomolecules analyzed by reversed-phase HPLC. The C4 bonded phase provides less hydrophobic retention than C8 and C18 phases, which can benefit large molecules that would otherwise be retained too strongly. The short 50 mm format allows rapid screening, method development, and routine bioanalytical workflows while maintaining reliable separation performance.
Key Benefits
RP C4™ Wide Pore stationary phase
USP L26 equivalent chemistry
Butyl bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
Optimized for proteins and peptides
Lower hydrophobic retention than C8 phases
5 µm particle size
Excellent for bioanalytical applications
Typical Applications
Protein analysis
Peptide analysis
Enzyme analysis
Bioanalytical methods
Biomolecule characterization
Pharmaceutical analysis
Biopharmaceutical analysis
Reversed-phase HPLC
Method development
High-throughput screening
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4™ Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve accessibility for larger analytes
Provides less retention than C8 and C18 stationary phases
Suitable for reversed-phase HPLC applications
The 50 mm length is ideal for rapid analyses and high-throughput workflows
5 µm particles provide low backpressure and robust operation
Fully double end capped to minimize secondary interactions
Frequently used for bioanalytical and biopharmaceutical applications
Excellent choice for protein and peptide method development
This is a Special Order Item