Catalog Number - 68004-25P

Cogent RP C4 Butyl is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. Classified as USP L26, this stationary phase provides moderate hydrophobic retention and is well suited for compounds that may be excessively retained on more hydrophobic phases such as C8 or C18 columns. The C4 chemistry offers reliable selectivity for hydrophobic and neutral analytes while maintaining shorter retention times and efficient chromatographic performance.

68004-25P is a 250 mm x 4.6 mm ID analytical HPLC column packed with 10 µm RP C4 Butyl particles featuring a 100 Å pore size. The extended 250 mm column length provides maximum resolving power, increased peak capacity, and enhanced separation of closely related compounds compared to shorter column formats. The 10 µm particle size supports higher flow rates with lower operating backpressure, while the 4.6 mm ID format is the industry standard for conventional HPLC systems and typically operates at approximately 1.0–2.0 mL/min.

This USP L26 column is particularly useful for hydrophobic and neutral compounds that require less retention than a comparable C8 phase. The combination of fully endcapped C4 chemistry, high-purity silica, larger particle technology, and extended column length delivers robust chromatographic performance for complex separations, impurity profiling, method optimization, and preparative method development.

Key Benefits

• RP C4 Butyl stationary phase

• USP L26 classification

• Fully double endcapped chemistry

• Traditional butyl (C4) bonded phase

• High-purity silica support

• 10 µm particles for lower backpressure

• Suitable for higher flow rates

• Less retention than C8 columns

• Maximum resolution from 250 mm column length

• Excellent for preparative method development

Typical Applications

• Reversed-phase HPLC

• USP L26 methods

• Hydrophobic compound analysis

• Neutral compound analysis

• Complex sample separations

• Impurity profiling

• Method development

• Preparative method scouting

• Scale-up studies

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double Endcapped

• Particle Size: 10 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Provides less retention than comparable C8 columns

• Suitable for hydrophobic and neutral compounds

• Larger particle size reduces operating backpressure

• Extended column length improves separation of closely related compounds

• Compatible with conventional HPLC systems

• Alternative for many USP L26 methods