Spironolactone USP assay with HPLC – INTERNAL ONLY
December 20, 2022
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CONFIDENTIAL – INTERNAL ONLY. Click HERE for public AppNote.
Alternate L1 column used for a USP assay of Spironolactone
This USP Spironolactone is easily performed, and the 10 injections overlay below demonstrates run to run consistency and great peak symmetry. The peak efficiency is very good and peak tailing guidelines are easily met with RSD values of less than 1.0%.
This demonstrates a good alternate column for your Spironolactone USP method.
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-15P
Dimensions: 4.6mm x 150mm
Mobile phase: Methanol and water (60:40)
Diluent: Acetonitrile and water (50:50)
Flow rate: 1.0mL / minute
Injection volume: 20 μL
Detection: UV @ 230nm
Sample Preparation: 0.5 mg/mL of Spironolactone in diluent
Tailing factor: NMT 2.0
Relative standard deviation: NMT 1.5%
Note 1: For the full method conditions and details, consult the official United States Pharmacopeia–National Formulary (USP–NF.)
Note 2: Spironolactone is a potassium-sparing diuretic (water pill). It prevents your body from absorbing too much salt and keeps your potassium levels from getting too low. This medicine is also used to treat or prevent hypokalemia (low potassium levels in the blood).
Alternate L1 column used for a USP assay of Spironolactone
This USP Spironolactone is easily performed, and the 10 injections overlay below demonstrates run to run consistency and great peak symmetry. The peak efficiency is very good and peak tailing guidelines are easily met with RSD values of less than 1.0%.
This demonstrates a good alternate column for your Spironolactone USP method.
Peak:
Spironolactone
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-15P
Dimensions: 4.6mm x 150mm
Mobile phase: Methanol and water (60:40)
Diluent: Acetonitrile and water (50:50)
Flow rate: 1.0mL / minute
Injection volume: 20 μL
Detection: UV @ 230nm
Sample Preparation: 0.5 mg/mL of Spironolactone in diluent
Tailing factor: NMT 2.0
Relative standard deviation: NMT 1.5%
Note 1: For the full method conditions and details, consult the official United States Pharmacopeia–National Formulary (USP–NF.)
Note 2: Spironolactone is a potassium-sparing diuretic (water pill). It prevents your body from absorbing too much salt and keeps your potassium levels from getting too low. This medicine is also used to treat or prevent hypokalemia (low potassium levels in the blood).