Retention of Erythromycin analyzed with ELSD - AppNote
June 16, 2022
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Erythromycin, a macrolide antibiotic
As this compound lacks chromophores, it typically requires derivatization for use in UV detection. In this method, we retain excellent peak shape without the need for these pre-column derivatization steps saving time and resources.
RSD values (less than 0.5%) demonstrate the consistent and reliable retention, as shown in the 10 injections overlay below.
Column: Cogent Diamond Hydride™, 4μm, 100Å.
Catalog No.: 70000-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 50% acetonitrile / 50% DI water / 0.1% formic acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute.
Detection: ELSD, gain: 9, temperature: 50°C, nitrogen: 3.5 bar.
Sample Preparation: 2.0mg / mL Erythromycin HCL in DI water.
Notes: Erythromycin is a macrolide antibiotic used to treat bacterial infections and is also used to prevent recurrent rheumatic fever.
As this compound lacks chromophores, it typically requires derivatization for use in UV detection. In this method, we retain excellent peak shape without the need for these pre-column derivatization steps saving time and resources.
RSD values (less than 0.5%) demonstrate the consistent and reliable retention, as shown in the 10 injections overlay below.
Peak:
Erythromycin
Column: Cogent Diamond Hydride™, 4μm, 100Å.
Catalog No.: 70000-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 50% acetonitrile / 50% DI water / 0.1% formic acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute.
Detection: ELSD, gain: 9, temperature: 50°C, nitrogen: 3.5 bar.
Sample Preparation: 2.0mg / mL Erythromycin HCL in DI water.
Notes: Erythromycin is a macrolide antibiotic used to treat bacterial infections and is also used to prevent recurrent rheumatic fever.