Ivermectin Analyzed with HPLC - AppNote
February 28, 2022
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A Robust Method for Analysis of an Antiparasitic Medication
A robust and reproducible Method has been developed for this anthelmintic agent. The data below (an overlay of 10 chromatograms ) illustrates both excellent Peak Shape and Retention using this easy Method.
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-15P
Dimensions: 4.6mm x 150mm
Mobile Phase:
--A: DI Water with 0.1% Formic Acid
--B: Acetonitrile with 0.1% Formic Acid
Injection vol.: 5μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Diluent: 50:50 DI Water / Acetonitrile
Sample Preparation: Ivermectin prepared as 6μg / mL Standard Solution in Diluent.
t0: 1.6 Minutes
K: 4.53
%RSD of 10 injections: ‹0.1%
Notes: Ivermectin is a semi-synthetic Antiparasitic Medication derived from Avermectins, a class of highly-active broad-spectrum Antiparasitic Agents isolated from the fermentation products of Streptomyces avermitilis. This drug is used in the treatment of Onchocerciasis, but may also be effective against other worm infestations (such as Strongyloidiasis, Ascariasis, Trichuriasis and Enterobiasis). Applied topically, it may be used in the treatment of head lice infestation.
Notes: Calculation for Capacity Factor - Relative Retention k = (tR-t0)/t0
A robust and reproducible Method has been developed for this anthelmintic agent. The data below (an overlay of 10 chromatograms ) illustrates both excellent Peak Shape and Retention using this easy Method.
Peak
1. Ivermectin
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-15P
Dimensions: 4.6mm x 150mm
Mobile Phase:
--A: DI Water with 0.1% Formic Acid
--B: Acetonitrile with 0.1% Formic Acid
| Time (minutes) | %B |
| 0 | 30 |
| 1 | 30 |
| 5 | 75 |
| 6 | 75 |
| 7 | 30 |
| 10 | 30 |
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Diluent: 50:50 DI Water / Acetonitrile
Sample Preparation: Ivermectin prepared as 6μg / mL Standard Solution in Diluent.
t0: 1.6 Minutes
K: 4.53
%RSD of 10 injections: ‹0.1%
Notes: Ivermectin is a semi-synthetic Antiparasitic Medication derived from Avermectins, a class of highly-active broad-spectrum Antiparasitic Agents isolated from the fermentation products of Streptomyces avermitilis. This drug is used in the treatment of Onchocerciasis, but may also be effective against other worm infestations (such as Strongyloidiasis, Ascariasis, Trichuriasis and Enterobiasis). Applied topically, it may be used in the treatment of head lice infestation.
Notes: Calculation for Capacity Factor - Relative Retention k = (tR-t0)/t0