Fluorouracil Assay Analyzed with HPLC – AppNote
August 17, 2021
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A Reliable Method for a Chemotherapeutic Drug
This Fluorouracil Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.1%. This Method shows reliability for analysis of this Pyrimidine Analog that is an anti-neoplastic anti-metabolite.
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-25P
Dimensions: 4.6mm x 250mm
Mobile Phase: (5:95) Methanol / Buffer
--Buffer: 0.73g / L of Monobasic Sodium Phosphate and 1.4g / L of Dibasic Sodium Phosphate in Water.
Injection vol.: 20μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Fluorouracil 1.0mg / mL in DI Water
Note: Fluorouracil is a Pyrimidine Analog used to treat Basal Cell Carcinomas, and as an injection in palliative Cancer treatment.
This Fluorouracil Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.1%. This Method shows reliability for analysis of this Pyrimidine Analog that is an anti-neoplastic anti-metabolite.
Peak:
Fluorouracil
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-25P
Dimensions: 4.6mm x 250mm
Mobile Phase: (5:95) Methanol / Buffer
--Buffer: 0.73g / L of Monobasic Sodium Phosphate and 1.4g / L of Dibasic Sodium Phosphate in Water.
Injection vol.: 20μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Fluorouracil 1.0mg / mL in DI Water
Note: Fluorouracil is a Pyrimidine Analog used to treat Basal Cell Carcinomas, and as an injection in palliative Cancer treatment.
This Method was developed by and is presented courtesy of ARL- Eutech Scientific Services, Inc.