Capillary conditioning in capillary electrophoresis - How To
November 29, 2016
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New capillaries should be conditioned before you use them.
1.Filter all solutions to be used on your capillary through a .45µ or 0.2µ syringe filter.
2.Treat the capillaries by running 0.1N NaOH solution through them on your CE unit.
3.Rinse the capillaries with CEwater or HPCE grade water for 5 minutes.
4.Final step is to run your buffer through it for 10 minutes before injection.
This procedure should be followed each day before new or old capillaries are used.
1.Filter all solutions to be used on your capillary through a .45µ or 0.2µ syringe filter.
2.Treat the capillaries by running 0.1N NaOH solution through them on your CE unit.
3.Rinse the capillaries with CEwater or HPCE grade water for 5 minutes.
4.Final step is to run your buffer through it for 10 minutes before injection.
This procedure should be followed each day before new or old capillaries are used.