Commercial Rice Extract Analyzed Easily.
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A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure.

Peaks:
1. 3,4-Hydroxybenzoic Acid 153 m/z [M-H]–
2. Vanillic Acid 167 m/z [M-H]–
3. p-Coumaric Acid 163 m/z [M-H]–
4. Ferulic Acid 193 m/z [M-H]–
5. 3,5-Dimethoxy-4-Hydroxycinnamic Acid 223 m/z [M-H]–

Method Conditions
Column: Cogent Phenyl Hydride™, 4µm, 1 00Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
- A: DI Water / 0.1% Formic Acid (v/v)
- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes)	%B
0	10
5	20
6	20
7	10

Post Time: 3 minutes
Injection vol.: 1µL
Flow rate: 0.4 mL/minute
Detection: ESI – NEG - PerkinElmer Flexar SQ 300 Mass Spectrometer
Samples:
- Figure A: Commercial rice extract was analyzed.
- Figure B: Peak of 3,5-Dimethoxy-4-Hydroxycinnamic Acid in commercial rice extract overlaid with the
- peak for 25 ppm standard.
t 0 : 0.4 minutes

Note: Rice is a staple food in many countries. It contains phenolic compounds which have anticancer, antioxidant, and antimutagenic effects. It is important to analyze rice extracts to confirm the content of the phenolic compounds in rice.



Attachment

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