Acrylamide HPLC Analyzed with HPLC - AppNote
August 13, 2012
/
/
/
/
/
/
Easy & Precise Retention of a Very Polar Compound
Acrylamide can be difficult to retain with conventional Reversed Phase Methods due to its polar nature. With this Method however, Retention is readily achievable using a simple, Isocratic Mobile Phase. The overlay of two Chromatograms using two different lots of HPLC Columns in the Figure below illustrates the Reproducibility and Robustness of this Method.
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: Acetonitrile with 0.1% Formic Acid
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 205nm
Sample Preparation: 0.1mg / mL Acrylamide in Mobile Phase as the Diluent.
t0: 1.0 minute
Note: Acrylamide is a monomer used to synthesize polyacrylamides. It was reported to be present in certain food products in 2002. This has been cause for concern as the monomer form is a known carcinogen and neurotoxin. As such, quantitation of this analyte is of importance in a variety of fields.
Attachment
No 199 Acrylamide Analyzed with HPLC pdf 0.3 Mb Download File
Acrylamide can be difficult to retain with conventional Reversed Phase Methods due to its polar nature. With this Method however, Retention is readily achievable using a simple, Isocratic Mobile Phase. The overlay of two Chromatograms using two different lots of HPLC Columns in the Figure below illustrates the Reproducibility and Robustness of this Method.
Peak:
Acrylamide
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: Acetonitrile with 0.1% Formic Acid
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 205nm
Sample Preparation: 0.1mg / mL Acrylamide in Mobile Phase as the Diluent.
t0: 1.0 minute
Note: Acrylamide is a monomer used to synthesize polyacrylamides. It was reported to be present in certain food products in 2002. This has been cause for concern as the monomer form is a known carcinogen and neurotoxin. As such, quantitation of this analyte is of importance in a variety of fields.
Attachment
No 199 Acrylamide Analyzed with HPLC pdf 0.3 Mb Download File