Fioricet Analyzed with HPLC - AppNote
May 16, 2012
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Gradient Separation of Acetaminophen, Caffeine, and Butalbital
In this Method, a simple Reversed Phase gradient is used to separate the three components of a Fioricet tablet. Figure A shows the five-run overlay obtained from the gradient. Figure B shows a “zoomed in view” so that the Butalbital Peak can be seen clearly.
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-15P
Dimensions: 4.6 x 150mm
Mobile Phase:
--A: DI Water / 0.1% Formic Acid
--B: 97% Acetonitrile / 3% DI Water / 0.1% Formic Acid
Gradient:
Temperature: 35˚C
Injection vol.: 10µL
Flow rate: 0.8mL / minute
Detection: UV @ 240nm
Sample Preparation:
--Stock Solution: Fioricet tablet was ground, added to 10mL volumetric flask and diluted with 50:50 Solvent A / Solvent B mixture. The flask was vortexed 5 minutes and a portion of the solution was filtered with a 0.45µm Nylon Syringe Filter.
--Working Solution: 10µL of the stock was diluted with 990µL of 50:50 Solvent A / Solvent B mixture.
t0: 2.2 minutes
Note: Fioricet/Esgic is a combination of Acetaminophen, Caffeine, and Butalbital. It is used to treat tension headaches, muscle contraction headaches, and migraines.
Attachment
No 137 Fioricet Analyzed with HPLC pdf 0.9 Mb Download File
In this Method, a simple Reversed Phase gradient is used to separate the three components of a Fioricet tablet. Figure A shows the five-run overlay obtained from the gradient. Figure B shows a “zoomed in view” so that the Butalbital Peak can be seen clearly.
1. Acetaminophen
2. Caffeine
3. Butalbital
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-15P
Dimensions: 4.6 x 150mm
Mobile Phase:
--A: DI Water / 0.1% Formic Acid
--B: 97% Acetonitrile / 3% DI Water / 0.1% Formic Acid
Gradient:
| Time (minutes) | %B |
| 0 | 10 |
| 6 | 70 |
| 7 | 10 |
Injection vol.: 10µL
Flow rate: 0.8mL / minute
Detection: UV @ 240nm
Sample Preparation:
--Stock Solution: Fioricet tablet was ground, added to 10mL volumetric flask and diluted with 50:50 Solvent A / Solvent B mixture. The flask was vortexed 5 minutes and a portion of the solution was filtered with a 0.45µm Nylon Syringe Filter.
--Working Solution: 10µL of the stock was diluted with 990µL of 50:50 Solvent A / Solvent B mixture.
t0: 2.2 minutes
Note: Fioricet/Esgic is a combination of Acetaminophen, Caffeine, and Butalbital. It is used to treat tension headaches, muscle contraction headaches, and migraines.
Attachment
No 137 Fioricet Analyzed with HPLC pdf 0.9 Mb Download File