L-a-phenylglycine Antibiotic Analysis with HPLC - AppNote
May 16, 2012
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Comparison using Cogent Silica-C™ and UDC-Cholesterol™ HPLC Columns
In this Method, a non-modified, silica-hydride HPLC Column, Cogent Silica-C, produces good Retention of the important amino acid L(+)-α-Phenylglycine with an Isocratic Mobile Phase. In addition the Retention Times are extremely Reproducible (%RSD 0.2).
The same Method using a Cogent UDC-Cholesterol Column (UDC), silica-hydride with an 11 Carbon Hydrocarbon and a Cholesterol molecule bonded to it, highlights the strong Retention on the Silica-C 4.6 x 75mm Column compared with the longer Cholesterol 4.6 x 150mm Column for this compound.
Both Chromatograms use the same Mobile Phase in the ANP or Aqueous Normal Phase mode of HPLC.
Method Conditions
Columns:
--Cogent Silica-C™, 4μm, 100Å
--Cogent UDC-Cholesterol, 4μm, 100Å
Catalog Nos.:
--40000-75P
--69069-15P
Dimensions:
--4.6 x 75mm
--4.6 x 150mm
Mobile Phase: A: 80% Acetonitrile / 19.5% DI Water / 0.5% Formic Acid (pre-mixed)
Injection vol.: 5μL
Flow rate: 1mL / minute
Detection: UV @ 254nm
Sample Preparation: L(+)-α-Phenylglycine 0.3mg / mL dissolved in the Mobile Phase
t0: 0.89 minutes
Note: Phenylglycine is a synthetic amino acid used in manufacturing Lactam antibiotics, such as semi synthetic Cephalosporins and Penicillins.
Attachment
No 46 L-a-phenylglycine Antibiotic Analysis with HPLC pdf 0.3 Mb Download File
In this Method, a non-modified, silica-hydride HPLC Column, Cogent Silica-C, produces good Retention of the important amino acid L(+)-α-Phenylglycine with an Isocratic Mobile Phase. In addition the Retention Times are extremely Reproducible (%RSD 0.2).
The same Method using a Cogent UDC-Cholesterol Column (UDC), silica-hydride with an 11 Carbon Hydrocarbon and a Cholesterol molecule bonded to it, highlights the strong Retention on the Silica-C 4.6 x 75mm Column compared with the longer Cholesterol 4.6 x 150mm Column for this compound.
Both Chromatograms use the same Mobile Phase in the ANP or Aqueous Normal Phase mode of HPLC.
Method Conditions
Columns:
--Cogent Silica-C™, 4μm, 100Å
--Cogent UDC-Cholesterol, 4μm, 100Å
Catalog Nos.:
--40000-75P
--69069-15P
Dimensions:
--4.6 x 75mm
--4.6 x 150mm
Mobile Phase: A: 80% Acetonitrile / 19.5% DI Water / 0.5% Formic Acid (pre-mixed)
Injection vol.: 5μL
Flow rate: 1mL / minute
Detection: UV @ 254nm
Sample Preparation: L(+)-α-Phenylglycine 0.3mg / mL dissolved in the Mobile Phase
t0: 0.89 minutes
Note: Phenylglycine is a synthetic amino acid used in manufacturing Lactam antibiotics, such as semi synthetic Cephalosporins and Penicillins.
Attachment
No 46 L-a-phenylglycine Antibiotic Analysis with HPLC pdf 0.3 Mb Download File