Flecainide Acetate USP assay with HPLC - INTERNAL ONLY
October 27, 2022
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CONFIDENTIAL – INTERNAL ONLY. Click here for public AppNote.
Alternate column used for a similar USP assay method of flecainide acetate
This Similar USP Flecainide Acetate is easily performed, and the 10 injections overlay below demonstrates run to run consistency and great peak symmetry. The peak efficiency is very good and peak tailing guidelines are easily met with RSD values of less than 1.0%.
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-15P
Dimensions: 4.6mm x 150mm
Mobile Phase:
—A: 0.1 M ammonium acetate in water. Adjust with acetic acid to a pH of 5.4.
B: Acetonitrile
Diluent: Acetonitrile and water (25:75)
Injection vol.: 10μL
Flow rate: 1.0mL / minute
Detection: UV @ 298nm
Column temperature: 25°C
Sample Preparation: 0.2 mg / mL of flecainide acetate in diluent
Tailing factor: NMT 2.0
Relative standard deviation: NMT 0.73%
Note 1: For the full method conditions and details, consult the official United States Pharmacopeia–National Formulary (USP–NF.)
Note 2: Flecainide Acetate inhibits the action of sodium and potassium ion channels in the heart, which raises the threshold for depolarization and correcting arrhythmias.
Alternate column used for a similar USP assay method of flecainide acetate
This Similar USP Flecainide Acetate is easily performed, and the 10 injections overlay below demonstrates run to run consistency and great peak symmetry. The peak efficiency is very good and peak tailing guidelines are easily met with RSD values of less than 1.0%.
This demonstrates a good alternate column for your Flecainide Acetate USP method.
Peak:
Flecainide Acetate
Column: Cogent RP C18™, 5um, 100Å
Catalog No.: 68518-15P
Dimensions: 4.6mm x 150mm
Mobile Phase:
—A: 0.1 M ammonium acetate in water. Adjust with acetic acid to a pH of 5.4.
B: Acetonitrile
| Time (minutes) | %B |
| 0 | 25 |
| 7 | 55 |
| 12 | 55 |
| 12.1 | 25 |
| 15 | 25 |
Injection vol.: 10μL
Flow rate: 1.0mL / minute
Detection: UV @ 298nm
Column temperature: 25°C
Sample Preparation: 0.2 mg / mL of flecainide acetate in diluent
Tailing factor: NMT 2.0
Relative standard deviation: NMT 0.73%
Note 1: For the full method conditions and details, consult the official United States Pharmacopeia–National Formulary (USP–NF.)
Note 2: Flecainide Acetate inhibits the action of sodium and potassium ion channels in the heart, which raises the threshold for depolarization and correcting arrhythmias.