Versatile Cyano HPLC Columns for USP® L10 Methods, RP, and NP Applications
Cogent Axis™ Cyano HPLC columns offer reliable, reproducible performance for a wide range of chromatographic methods, including USP® L10 applications. These columns can be used in both reversed‑phase (RP) and normal‑phase (NP) separations, providing flexibility for method development and routine analysis.
As one of MICROSOLV’s original column technologies, Cogent Axis™ Cyano remains in active use in laboratories worldwide. This proven platform also served as the foundation for the later Cogent RP™ and Cogent NP™ product lines, which incorporate narrower particle size distributions and additional performance enhancements.
Proven Cyano Chemistry on High‑Purity Silica
Cogent Axis™ Cyano columns are non‑end capped and manufactured using 100 Å, high‑purity silica, bonded with a cyano (CN) ligand. This surface chemistry provides balanced polarity, making the column suitable for both RP and NP modes while maintaining consistent selectivity across a broad range of analytes.
Available particle sizes include:
3 µm silica – optimized for higher efficiency and resolution
5 µm silica – ideal for robust, routine analytical methods
This chemistry is especially well suited for laboratories seeking a dependable cyano phase with well‑established performance characteristics.
Key Features and Benefits
Designed for USP® L10 methods
Suitable for reversed‑phase and normal‑phase HPLC
Non‑end capped cyano (CN) bonded phase
Manufactured with 100 Å, high‑purity silica
Available in 3 µm and 5 µm particle sizes
Proven, long‑standing column technology
Reliable performance for routine and legacy methods
Typical Applications
USP® L10 compliant methods
Polar and moderately polar compound separations
Method development requiring RP/NP flexibility
Legacy methods originally developed on cyano phases
Routine quality control and analytical testing
Column Protection Recommendation
Important: MICROSOLV technical support strongly recommends the use of the Cogent column filter to protect your column, which is included with each column, whenever possible. Using a column filter helps protect the analytical column from particulates, extends column lifetime, and supports consistent performance.
