Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 14, 2020
Bill Ciccone

2.o™ HPLC Columns FAQ / ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Diamond Hydride FAQ / Frequently Asked Questions?? / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Phenyl Hydride FAQ / TYPE-C in General FAQ / UDC-Cholesterol FAQ

Methanol is generally not recommended for most ANP applications however a few scientists have seen success with it.

Instead, it is best to use polar aprotic solvents such as acetonitrile. If using ELSD or LCMS, acetone can also be a good alternative to acetonitrile as well. However, because of its low UV cutoff, it is generally not appropriate for analyses performed with UV detection.

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