Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 14, 2020
Bill Ciccone

2.o™ HPLC Columns FAQ / ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Bidentate C18 FAQ / Bidentate C8 FAQ / Diamond Hydride FAQ / Diol FAQ / Frequently Asked Questions?? / How To... Information / HPLC Column Information / HPLC Columns FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Phenyl Hydride FAQ / Trouble Shooting Suggestions / TYPE-C Column Information / TYPE-C in General FAQ / UDC-Cholesterol FAQ

Suggested Procedure:

A – Changing from Reversed Phase to Normal Phase HPLC; pump 100% methanol for 15 minutes at 1 mL / minute flow rate, followed by 15 minutes 100% methylene chloride. The column is ready to be equilibrated with mobile phase for NP HPLC.

B – Changing from Normal Phase to Reverses Phase HPLC; pump 100% methylene chloride for 15 minutes at 1 mL / minute flow rate, followed by 15 minutes 100% methanol. The column is ready to be equilibrated with mobile phase for RP-HPLC.

A simple 30 minute procedure allows switching from one mode to another.

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