Can I use the HSQ Kit with the longer column from the original PQ Kit – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 15, 2014
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Column Information / HPLC Columns FAQ / HPLC Qualification / Qualification FAQ / Qualification Information

If you’re required to use the older 150mm PQ Kit™ column and cannot use the 75mm HSQ Kit™ column, you can adjust the parameters in the Excel spreadsheet shown here:

Change Flow Rate = 1 mL/minute
Change Expected Retention Times/Run Times = 4x longer than in the Excel sheet to compensate for lower flow rate (see above) and longer column length. You can simply adjust the Excel sheet cells for this.
Change Injection Volume: Increase from 8uL to 12uL to compensate for larger peaks.
Change Gradient Test: If you have an older system with large dwell volume, you may want to change the steps in the gradient from 5 minute steps to 10 minute steps.

All other test parameters and results are not affected by this and remain the same between both kits.

Using the 75mm column is recommended when possible as it will save you 75% of the time to run these tests.

Click HERE for HSQ & PQ Kits ordering information and pictures

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Related Articles

1) What is the difference between the HSQ Kit and older PQ Kit – FAQ

2) Can the HSQ or PQ qualification kits be used with Waters equipment and Empower chromatography data software – FAQ

3) How does the shorter column supplied in the HSQ kit affect the data compared to the longer column from the PQ Kit – FAQ

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