Catalog Number - 69269-05P-2

The 69269‑05P‑2 Cogent UDC‑Cholesterol 2.0 HPLC Column delivers unique shape‑based selectivity using 2.2 µm TYPE‑C™ silica hydride particles functionalized with a cholesterol‑terminated ligand.

This stationary phase consists of an 11‑carbon chain bonded directly to silica hydride with a cholesterol group at the terminus, creating structured interaction regions that form “molecular slots.” These allow compounds to be separated not only by hydrophobicity, but by molecular shape and steric fit, providing selectivity unavailable from traditional reversed‑phase columns.

Unlike conventional silica phases:

• No end‑capping is required (direct Si–C bond)

• Reduced secondary interactions

• Improved reproducibility and stability

This column supports multiple separation modes:

• Reversed Phase

• Normal Phase

• HILIC

50 mm Length – Speed & Throughput

• Optimized for rapid separations and short run times

• Lower backpressure compared to longer columns

• Ideal for high‑throughput screening and fast LC‑MS analysis

• Supports efficient method development workflows

Flow Rate & System Requirements (2.1 mm ID)

Requires precise low‑flow UHPLC capability

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.25–0.35 mL/min

• Requires:

  • UHPLC system capable of stable, low‑flow delivery

  • Low‑volume flow cell detector

  • Accurate, low‑volume injection system

⚠️ Standard HPLC systems must be capable of reliable low‑flow operation to achieve proper performance.

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D structure and steric interactions

• Excellent for isomers and structurally similar compounds

Highly Hydrophobic Retention

• Strong interaction with hydrophobic analytes

• Improved retention for difficult compounds

Flexible Selectivity Control

• Selectivity can be tuned by:

  • Temperature changes

  • Mobile phase composition

• Enables fine control over separation conditions

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Rapid re‑equilibration between runs

• Increased analytical throughput vs conventional RP and HILIC phases

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across multiple solvent systems

• Long operational lifetime under demanding conditions

Typical Applications

• Steroidal compound analysis

• Structural isomer separations

• Lipids and highly hydrophobic molecules

• Peptides, proteins, and oligonucleotides

• Compounds difficult to retain or resolve on conventional RP columns

Important Usage Notes

• Designed for UHPLC / low‑flow applications

• Requires precision pumping and low‑volume detection systems

• Use temperature and solvent optimization to tune selectivity

• Ideal when traditional C18/C8 phases cannot resolve isomers