Catalog Number - 69069-10P

The 69069‑10P Cogent UDC‑Cholesterol HPLC Column provides unique shape‑based selectivity using 4 µm TYPE‑C™ silica hydride particles functionalized with a cholesterol‑terminated ligand.

This stationary phase features an 11‑carbon chain directly bonded to silica hydride with a cholesterol group at the terminus, creating structured interaction zones that act as molecular “slots.” These enable separations based on molecular geometry and steric fit, not just hydrophobic interactions—delivering selectivity beyond conventional reversed‑phase columns.

Unlike traditional silica phases:

• Direct Si–C bonding eliminates the need for end‑capping

• Reduced silanol activity

• Improved reproducibility and stability

This column is compatible with multiple separation modes:

• Reversed Phase

• Normal Phase

• HILIC

100 mm Length – Balanced Resolution & Throughput

• Increased resolving power vs 50 mm columns

• Improved isomer separation and peak capacity

• Ideal when 50 mm columns are too fast but lack resolution

• Moderate run time with strong analytical performance

This configuration is commonly selected for routine analytical methods requiring reliable resolution without long cycle times.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard column size

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Robust detector response

• High reproducibility across instruments

• Easy method transfer and scalability

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D molecular structure

• Ideal for isomers and closely related analytes

Very Hydrophobic Phase

• Strong interaction with lipids and hydrophobic compounds

• Effective for difficult‑to‑retain analytes

Tunable Selectivity

• Adjust separation behavior using:

  • Temperature

  • Mobile phase composition

• Enables advanced method development

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping required)

• Reduced secondary interactions

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Faster than conventional reversed‑phase or HILIC columns

• Reduced cycle time between runs

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across wide solvent conditions

• Long operational durability

Typical Applications

• Steroidal compound analysis

• Structural isomer separations

• Lipids and hydrophobic analytes

• Peptides, proteins, and oligonucleotides

• Routine analytical workflows

• Methods where C18 or C8 selectivity is insufficient

Important Usage Notes

• Ideal for standard HPLC systems (no special equipment required)

• Perfect balance between speed (50 mm) and resolution (longer columns)

• Optimize temperature and solvent composition for selectivity control

• Preferred when moderate-to-high resolution is required without long run times