Catalog Number - 69069-05P-2

The 69069‑05P‑2 Cogent UDC‑Cholesterol HPLC Column provides unique shape‑based selectivity using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.

This phase consists of an 11‑carbon chain bonded directly to silica hydride with a cholesterol moiety at the terminus, forming structured interaction regions that act as molecular “slots.” These enable separations based on molecular geometry and steric fit, not just hydrophobic interaction—delivering selectivity that conventional reversed‑phase columns cannot achieve.

Unlike traditional silica phases:

• Direct Si–C bonding eliminates the need for end‑capping

• Reduced silanol interactions

• Improved reproducibility and robustness

This column can be used in multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

50 mm Length – Speed & LC‑MS Throughput

• Optimized for rapid separations and short run times

• Lower backpressure than longer analytical columns

• Ideal for LC‑MS screening and high‑throughput workflows

• Preferred for method development and fast analysis

Flow Rate & System Requirements (2.1 mm ID, 4 µm)

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.25–0.35 mL/min

• Requires:

  • Reliable low‑flow HPLC or LC‑MS system

  • Low‑volume detector flow cell for best sensitivity

  • Precision pumping for stable flow delivery

⚠️ While compatible with standard HPLC systems, performance depends on the system’s ability to maintain stable low flow rates.

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D molecular structure

• Ideal for isomer and sterically similar analytes

Strong Hydrophobic Retention

• Highly hydrophobic stationary phase

• Retains lipids and challenging compounds effectively

Tunable Selectivity

• Separation can be modified using:

  • Temperature changes

  • Solvent composition

• Enables advanced method development flexibility

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol activity

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Rapid re‑equilibration vs conventional RP or HILIC columns

• Increased throughput

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across varied mobile phase conditions

• Long operational durability

Typical Applications

• Steroidal compound analysis

• Lipids and hydrophobic molecules

• Peptides, proteins, and oligonucleotides

• Structural isomer separations

• LC‑MS workflows requiring fast analysis and unique selectivity