Catalog Number - 69069-05P

The 69069‑05P Cogent UDC‑Cholesterol HPLC Column delivers unique shape‑based selectivity using 4 µm TYPE‑C™ silica hydride particles modified with a cholesterol‑terminated ligand.

This stationary phase consists of an 11‑carbon chain bonded directly to silica hydride with a cholesterol moiety at the terminus, forming structured interaction environments that act as molecular “slots.” These enable separations based on molecular shape, steric orientation, and geometry, not just hydrophobicity—providing selectivity not achievable with conventional reversed‑phase columns.

Unlike traditional silica:

• Direct Si–C bonding eliminates the need for end‑capping

• Reduced silanol activity and secondary interactions

• Improved reproducibility and robustness

This column supports multiple chromatographic modes:

• Reversed Phase

• Normal Phase

• HILIC

50 mm Length – Speed & High‑Throughput Screening

• Optimized for short run times and rapid separations

• Lower backpressure vs longer columns

• Ideal for high‑throughput screening (HTS)

• Supports fast method development and routine analysis

4.6 mm ID – Standard Analytical Performance

• Industry‑standard column dimension

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong signal response with standard detectors

• Robust performance across a wide range of applications

• Easy method transfer and scalability

Why Choose UDC‑Cholesterol™

Shape & Isomer Selectivity

• Separates compounds based on 3D structure and steric fit

• Ideal for isomers and structurally similar analytes

Very Hydrophobic Phase

• Strong retention of lipids and hydrophobic compounds

• Effective for difficult‑to‑retain analytes

Tunable Selectivity

• Selectivity can be modified using:

  • Temperature changes

  • Mobile phase composition

• Enables flexible method optimization

Cogent TYPE‑C™ Technology Advantages

Silica Hydride Surface

• Direct Si–C bonding (no end‑capping required)

• Reduced silanol interactions

• Improved peak shape for basic and polar compounds

Faster Equilibration

• Faster than conventional RP and HILIC columns

• Reduced run‑to‑run cycle time

Robust Column Lifetime

• Resistant to hydrolysis

• Stable across various mobile phases

• Long operational durability

Typical Applications

• Steroidal compound analysis

• Structural isomer separations

• Lipids and hydrophobic compounds

• Peptides, proteins, and oligonucleotides

• High‑throughput screening workflows

• Methods where C18/C8 selectivity is insufficient