This items is made to order and is not returnable and order cannot be canceled.

CUSTOM ITEM: HPLC Column, Diamond Hydride, 4 um, 2.1 mm ID x 100 mm Length, 100 A. Packed in a "metal free" Stainless Steel Column and Frits. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 70000‑10D‑2 Diamond Hydride HPLC Column is a bio‑inert, metal‑free analytical column specifically designed for LC‑MS, HILIC, and mixed‑mode separations of polar and metal‑sensitive analytes. Manufactured using advanced Cogent TYPE‑C™ silica hydride technology, this column delivers excellent retention, very fast equilibration, and long‑term reproducibility.

The 2.1 mm × 100 mm format provides increased resolving power compared to shorter columns while maintaining LC‑MS‑appropriate flow rates. The permanently applied metal‑free surface coating shields analytes from unwanted interactions with stainless steel hardware, improving data quality for sensitive compounds.

This item is custom and can be manufactured to order.

Why This Column Is Used

The 2.1 mm internal diameter is widely adopted for LC‑MS workflows where sensitivity, solvent efficiency, and system compatibility are critical. This format offers:

  • Reduced solvent consumption compared to standard analytical columns

  • Improved MS sensitivity due to lower volumetric flow

  • Increased resolution versus 50 mm columns

  • Compatibility with MS detectors and modern UHPLC systems

The 100 mm length further enhances separation efficiency for complex polar mixtures without excessive run times.

Diamond Hydride™ Stationary Phase Advantage

Manufactured with Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase provides key advantages over conventional bonded‑silica HILIC materials:

  • Very fast equilibration, reducing method cycle time

  • Stable, reproducible retention across a wide range of mobile‑phase compositions

  • Long column lifetime under demanding LC‑MS conditions

The silica hydride surface supports mixed‑mode retention, making it especially well suited for compounds that are difficult to analyze using traditional reversed‑phase columns.

Metal‑Free, Bio‑Inert Hardware

The metal‑free stainless steel column and frit design permanently isolates analytes from exposed metal surfaces. This is particularly important for:

  • Proteins and peptides

  • Phosphorylated or chelating compounds

  • Trace‑level and metal‑sensitive small molecules

The result is improved peak shape, reduced adsorption, and enhanced data accuracy and reproducibility.

Typical Applications

This column is routinely used for:

  • Metabolomics and bioanalytical assays

  • Amino acids and organic acids

  • Polar and mixed‑polarity peptides

  • Complex biological matrices

  • LC‑MS workflows requiring increased resolution

High‑Resolution LC‑MS Performance

The 100 mm column length increases plate count and separation efficiency, making this column effective for:

  • LC‑MS methods where additional resolution is required

  • Complex sample matrices

  • Applications where peak capacity is more important than absolute speed

The narrow internal diameter supports enhanced MS response while maintaining manageable system pressures.

System Requirements

  • Typical operating flow rates: ~0.2–0.3 mL/min, depending on method and solvent viscosity

  • System must be capable of stable, repeatable low‑flow operation

  • Compatible with modern LC‑MS and UHPLC systems designed for narrow‑bore columns

Flow rates may be optimized further based on resolution goals, pressure limits, and MS interface requirements.

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