Catalog Number - 68524-SP100-3M

Cogent RP Phenyl™ Wide Pore Semi Prep HPLC Columns are designed for purification, method scale-up, and semi-preparative chromatography involving larger aromatic compounds and structurally related analytes. Manufactured using ultra-high purity silica bonded with a three-carbon linker and terminal Phenyl functionality, this stationary phase is fully double end-capped to minimize secondary interactions and provide highly reproducible chromatographic performance. The 300Å wide pore structure improves accessibility for larger molecules while maintaining the aromatic selectivity characteristic of RP Phenyl™ chemistry.

68524-SP100-3M is packed with 5 µm RP Phenyl™ Wide Pore particles featuring a 300Å pore size in a 10 mm ID x 100 mm semi-preparative format. The 5 µm particle size performs well at higher flow rates while maintaining moderate operating backpressure. The larger internal diameter provides increased loading capacity compared to analytical columns, making this column ideal for purification, fraction collection, and analytical-to-semi-preparative method scale-up.

The 10 mm ID x 100 mm format is particularly well suited for purification of larger positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, peptides, and related compounds containing aromatic structures. Typical operating flow rates are approximately 4–8 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A semi-preparative HPLC system capable of supporting these flow rates and sample loads is required.

Key Benefits

• RP Phenyl™ Wide Pore stationary phase

• USP L11 equivalent chemistry

• Ultra-high purity silica support

• Fully double end-capped

• Phenyl bonded phase

• 300Å pore size

• Suitable for reversed-phase chromatography

• Enhanced aromatic selectivity

• Improved accessibility for larger molecules

• Increased sample loading capacity

Typical Applications

• Semi-preparative HPLC

• Purification of larger positional isomers

• Tocopherol purification

• Flavonoid purification

• Polynuclear aromatic hydrocarbon separations

• Nitro-aromatic compound purification

• Active pharmaceutical ingredient (API) purification

• Natural product isolation

• Peptide purification

• Analytical-to-semi-preparative method scale-up

Specifications

• Product Type: Semi Prep HPLC Column

• Stationary Phase: RP Phenyl™ Wide Pore

• Chromatography Mode: Reversed Phase

• USP Classification: L11

• Particle Size: 5 µm

• Pore Size: 300Å

• Column Dimensions: 10 mm ID x 100 mm

• Recommended Flow Rate: 4–8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for reversed-phase chromatography

• USP L11 equivalent chemistry

• Fully double end-capped stationary phase

• Wide pore design improves accessibility for larger molecules

• Excellent for purification of peptides, natural products, APIs, and other large aromatic compounds

• Increased loading capacity compared to analytical columns

• Typical operating range is approximately 4–8 mL/min

• Requires a semi-preparative HPLC system capable of reliable operation at semi-prep flow rates

• Well suited for purification, fraction collection, and method scale-up

• This is a Special Order Item