Catalog Number - 70000-P30-100

The 70000‑P30‑100 Diamond Hydride Preparative HPLC Column is designed for process‑oriented preparative purification where both high sample capacity and improved chromatographic resolution are required. This mid‑length, large‑diameter configuration provides greater resolving power than short 50 mm columns while maintaining throughput suitable for purification workflows supporting downstream research or commercial objectives.

Preparative Use Case: Why 30 mm ID × 100 mm

The 30 mm internal diameter classifies this column as true process‑scale preparative, intended for laboratories handling large injection volumes and collecting substantial quantities of purified analytes.

The 100 mm column length provides a practical balance between capacity and resolution:

• Increased separation efficiency versus 50 mm preparative columns

• Improved resolution of partially overlapping analytes

• Maintainable backpressure at very high flow rates

• Efficient throughput for repeated purification cycles

This configuration is typically selected when short columns lack sufficient resolution, but full‑length 150–250 mm preparative columns are unnecessary or less efficient for the process.

Diamond Hydride™ TYPE‑C Stationary Phase Advantages

Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase offers distinct performance advantages for preparative‑scale HILIC separations:

• Stable, reproducible retention of polar and basic compounds

• Faster equilibration compared to conventional bonded‑silica HILIC phases

• Excellent chemical stability in high‑organic mobile phases

• Long service life in continuous or repetitive purification workflows

This stationary phase is particularly effective for purification of:

• Peptides and peptide‑like analytes

• Metabolites and highly polar small molecules

• Amine‑containing and basic compounds

• Targets best isolated via HILIC‑based retention mechanisms

Purpose‑Built for Process & Production‑Oriented Purification

The 70000‑P30‑100 enables predictable scale‑up from smaller preparative formats while preserving chromatographic behavior. It is commonly used in process development, pilot‑scale purification, and production‑support workflows where purified analytes are required for continued research, formulation, or sale.

System Requirements

Typical operating flow rates: ~40–70 mL/min

This column requires a dedicated preparative or process‑scale HPLC system capable of:

• Sustained high‑flow solvent delivery

• Large volume injections

• Pressure limits compatible with longer preparative columns

• Appropriate detector configuration and fraction collection hardware

Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.

Flow rates should be optimized based on solvent composition, viscosity, pressure limitations, and process objectives.

Made‑to‑Order Columns & Pricing Information

These columns represent a significant investment and are frequently application‑specific. To ensure customers receive the correct column for their workflow:

• Pricing and technical support are provided through our Customer Service Department via formal quotation

• Columns are produced on order, based on customer requirements

• Orders cannot be canceled or returned once placed

• Learn more about Custom Orders and our Custom Order policies.