Catalog Number - 82561-P50-1003M

Cogent NP Silica™ Wide Pore Process HPLC Columns are designed for large-scale purification, process chromatography, and production applications involving larger polar and hydrophilic compounds. Manufactured from ultra-high purity, low-metal-content 300 Å silica, this stationary phase is completely unmodified, providing the traditional selectivity associated with USP L3 classified silica while improving accessibility for larger molecular weight analytes. NP Silica™ Wide Pore is widely used for purification of proteins, peptides, lipids, and other large polar compounds in preparative and process-scale environments.

82561-P50-1003M is packed with 5 µm NP Silica™ Wide Pore particles in a 50 mm ID x 100 mm process-scale format. The 300 Å pore structure enhances mass transfer and analyte accessibility for larger molecules, while the 5 µm particle size supports high process flow rates with lower operating backpressures than smaller particles. The large internal diameter provides extremely high sample loading capacity, making this column ideal for production-scale purification, fraction collection, and process development.

The 50 mm ID x 100 mm format is well suited for process HILIC and normal-phase chromatography involving proteins, peptides, lipids, and other large hydrophilic or polar compounds. Typical flow rates are approximately 80-140 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative or process HPLC system capable of high flow rates and large-volume sample loading is required for optimum performance.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity, low-metal-content silica

• Unmodified silica surface

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Enhanced accessibility for larger molecules

• Extremely high sample loading capacity

• Lower backpressure with 5 µm particles

Typical Applications

• Process HILIC chromatography

• Process normal-phase HPLC

• Protein purification

• Peptide purification

• Lipid purification

• Biomolecule isolation

• Polar compound purification

• Production-scale separations

• Process development

• Method scale-up

Specifications

• Product Type: Process HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• USP Classification: L3

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 50 mm ID x 100 mm

• Recommended Flow Rate: 80-140 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Unmodified silica stationary phase

• USP L3 equivalent chemistry

• Wide pore design improves accessibility for proteins, peptides, lipids, and larger molecules

• Excellent for biomolecule and high molecular weight compound purification

• Large 50 mm ID provides extremely high sample loading capacity

• 100 mm length offers an excellent balance of throughput and resolution

• Typical operating range is approximately 80-140 mL/min

• Requires a specialized preparative or process HPLC system capable of high flow rates

• Ideal for production-scale purification, fraction collection, and process development

• This is a Special Order Item