Catalog Number - 82560-SP50

Cogent NP Diol™ Semi-Preparative HPLC Columns are designed for purification, method scale-up, and semi-preparative chromatography applications involving polar and hydrophilic compounds. Manufactured using ultra-high purity silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent durability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for established methods and semi-preparative method development.

82560-SP50 is packed with 5 µm NP Diol™ particles featuring a 100 Å pore size in a 10 mm ID x 50 mm semi-preparative format. The larger internal diameter provides increased sample loading capacity compared to analytical columns, while the short column length is ideal for rapid purification workflows and reduced solvent consumption. NP Diol™ is frequently selected as an alternative to unmodified silica columns because it often offers improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 10 mm ID x 50 mm format is well suited for rapid semi-preparative HILIC and normal-phase chromatography applications involving hydrophilic and polar compounds. Typical flow rates are approximately 4-8 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A semi-preparative HPLC system capable of higher flow rates and increased sample loading is recommended for optimum performance.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity silica support

• Terminal diol functional group

• 100 Å pore size

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Alternative to unmodified silica columns

• Increased sample loading capacity

• Broad solvent compatibility

Typical Applications

• Semi-preparative HILIC chromatography

• Semi-preparative normal-phase HPLC

• Compound purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Polar compound isolation

• Hydrophilic compound purification

• Process development

• Alternative USP L20 separations

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: NP Diol™

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 10 mm ID x 50 mm

• Recommended Flow Rate: 4-8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC and normal-phase semi-preparative chromatography

• Often used as an alternative to USP L20 columns

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• 10 mm ID format provides increased sample loading capacity

• Short 50 mm length minimizes run times and solvent consumption

• Typical operating range is approximately 4-8 mL/min

• Requires a semi-preparative HPLC system capable of higher flow rates and sample loading

• Ideal for purification, fraction collection, and method scale-up

• This is a Special Order Item