Catalog Number - 82560-SP100-3M

Cogent NP Diol™ Wide Pore Semi-Preparative HPLC Columns are designed for purification, method scale-up, and semi-preparative chromatography applications involving larger polar and hydrophilic molecules. Manufactured using ultra-high purity 300 Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent durability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for established methods and semi-preparative method development involving larger molecular weight analytes.

82560-SP100-3M is packed with 5 µm NP Diol™ Wide Pore particles featuring a 300 Å pore size in a 10 mm ID x 100 mm semi-preparative format. The wide pore structure improves accessibility for proteins, peptides, and other larger molecules while maintaining excellent retention of hydrophilic and polar compounds. The larger internal diameter provides increased sample loading capacity compared to analytical columns, making it ideal for compound isolation, fraction collection, and analytical-to-preparative method scale-up. NP Diol™ Wide Pore columns are frequently selected as alternatives to unmodified silica columns because they often provide improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 10 mm ID x 100 mm format is well suited for semi-preparative HILIC and normal-phase chromatography applications involving proteins, peptides, and other biomolecules. Typical flow rates are approximately 4-8 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A semi-preparative HPLC system capable of higher flow rates and increased sample loading is recommended for optimum performance.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity 300 Å silica support

• Terminal diol functional group

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Ideal for proteins, peptides, and larger molecules

• Increased sample loading capacity

• Broad solvent compatibility

Typical Applications

• Semi-preparative HILIC chromatography

• Semi-preparative normal-phase HPLC

• Protein purification

• Peptide purification

• Biomolecule isolation

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Polar compound purification

• Process development

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 10 mm ID x 100 mm

• Recommended Flow Rate: 4-8 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC and normal-phase semi-preparative chromatography

• Often used as an alternative to USP L20 columns

• Wide pore design improves accessibility for proteins, peptides, and larger molecules

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• 10 mm ID format provides increased sample loading capacity

• 100 mm length offers an excellent balance of throughput and resolution

• Typical operating range is approximately 4-8 mL/min

• Requires a semi-preparative HPLC system capable of higher flow rates and sample loading

• Ideal for purification, fraction collection, and method scale-up of biomolecules

• This is a Special Order Item