Catalog Number - 82560-P50-1503M

Cogent NP Diol™ Wide Pore Process HPLC Columns are designed for large-scale purification, process development, and production-scale chromatography involving larger polar and hydrophilic molecules. Manufactured using ultra-high purity 300 Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent durability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for established methods and process-scale method development involving larger molecular weight analytes.

82560-P50-1503M is packed with 5 µm NP Diol™ Wide Pore particles featuring a 300 Å pore size in a 50 mm ID x 150 mm process-scale format. The wide pore structure improves accessibility for proteins, peptides, and other higher molecular weight compounds while maintaining excellent retention of hydrophilic and polar compounds. NP Diol™ Wide Pore columns are frequently selected as alternatives to unmodified silica columns because they often provide improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 50 mm ID x 150 mm format is ideal for process-scale HILIC and normal-phase chromatography applications requiring increased resolving power and exceptional sample loading capacity. Typical flow rates are approximately 80-140 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized process or preparative HPLC system capable of high flow rates and large sample loading is required for optimum performance.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity 300 Å silica support

• Terminal diol functional group

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Ideal for proteins, peptides, and larger molecules

• Exceptional sample loading capacity

• Broad solvent compatibility

Typical Applications

• Process HPLC

• Preparative HILIC chromatography

• Preparative normal-phase HPLC

• Protein purification

• Peptide purification

• Biomolecule isolation

• Production-scale chromatography

• Fraction collection

• Process development

• Pharmaceutical purification

Specifications

• Product Type: Process HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 50 mm ID x 150 mm

• Recommended Flow Rate: 80-140 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC and normal-phase process chromatography

• Often used as an alternative to USP L20 columns

• Wide pore design improves accessibility for proteins, peptides, and larger molecules

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• 50 mm ID format provides exceptional sample loading capacity

• 150 mm length provides increased resolution and purification efficiency

• Typical operating range is approximately 80-140 mL/min

• Requires a specialized process or preparative HPLC system capable of high flow rates and sample loading

• Ideal for large-scale purification, fraction collection, and process development

• This is a Special Order Item