Catalog Number - 82560-P30-2503M

Cogent NP Diol™ Wide Pore Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications involving larger polar and hydrophilic molecules. Manufactured using ultra-high purity 300 Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent durability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for established methods and preparative method development involving larger molecular weight analytes.

82560-P30-2503M is packed with 5 µm NP Diol™ Wide Pore particles featuring a 300 Å pore size in a 30 mm ID x 250 mm preparative format. The wide pore structure improves accessibility for proteins, peptides, and other higher molecular weight compounds while maintaining excellent retention of hydrophilic and polar compounds. NP Diol™ Wide Pore columns are frequently selected as alternatives to unmodified silica columns because they often provide improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 30 mm ID x 250 mm format is ideal for demanding preparative HILIC and normal-phase chromatography applications requiring maximum resolving power and very high sample loading capacity. Typical flow rates are approximately 30-50 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative HPLC system capable of high flow rates and large sample loading is required for optimum performance.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity 300 Å silica support

• Terminal diol functional group

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Ideal for proteins, peptides, and larger molecules

• Very high sample loading capacity

• Broad solvent compatibility

Typical Applications

• Preparative HILIC chromatography

• Preparative normal-phase HPLC

• Protein purification

• Peptide purification

• Biomolecule isolation

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Polar compound purification

• Process development

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 30 mm ID x 250 mm

• Recommended Flow Rate: 30-50 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC and normal-phase preparative chromatography

• Often used as an alternative to USP L20 columns

• Wide pore design improves accessibility for proteins, peptides, and larger molecules

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• 30 mm ID format provides very high sample loading capacity

• 250 mm length provides maximum resolution and purification efficiency

• Typical operating range is approximately 30-50 mL/min

• Requires a specialized preparative HPLC system capable of high flow rates and sample loading

• Ideal for purification, fraction collection, and method scale-up of biomolecules

• This is a Special Order Item