Catalog Number - 82560-P21-50-3M

Cogent NP Diol™ Wide Pore Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications involving larger polar and hydrophilic molecules. Manufactured using ultra-high purity 300 Å silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent durability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for established methods and preparative method development involving larger molecular weight analytes.

82560-P21-50-3M is packed with 5 µm NP Diol™ Wide Pore particles featuring a 300 Å pore size in a 21.2 mm ID x 50 mm preparative format. The wide pore structure improves accessibility for proteins, peptides, and other higher molecular weight compounds while maintaining excellent retention of hydrophilic and polar compounds. The short column length minimizes run times and solvent consumption while maintaining substantial sample loading capacity. NP Diol™ Wide Pore columns are frequently selected as alternatives to unmodified silica columns because they often provide improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 21.2 mm ID x 50 mm format is ideal for rapid preparative HILIC and normal-phase chromatography applications involving proteins, peptides, and other larger molecules. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative HPLC system capable of high flow rates and large sample loading is required for optimum performance.

Key Benefits

• NP Diol™ Wide Pore stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity 300 Å silica support

• Terminal diol functional group

• 300 Å wide pore structure

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Ideal for proteins, peptides, and larger molecules

• High sample loading capacity

• Broad solvent compatibility

Typical Applications

• Preparative HILIC chromatography

• Preparative normal-phase HPLC

• Protein purification

• Peptide purification

• Biomolecule isolation

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Polar compound purification

• Process development

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: NP Diol™ Wide Pore

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 21.2 mm ID x 50 mm

• Recommended Flow Rate: 15-25 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC and normal-phase preparative chromatography

• Often used as an alternative to USP L20 columns

• Wide pore design improves accessibility for proteins, peptides, and larger molecules

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• 21.2 mm ID format provides substantial sample loading capacity

• Short 50 mm length minimizes run times and solvent consumption

• Typical operating range is approximately 15-25 mL/min

• Requires a specialized preparative HPLC system capable of high flow rates and sample loading

• Ideal for purification, fraction collection, and method scale-up of biomolecules

• This is a Special Order Item