Catalog Number - 82560-P21-100

Cogent NP Diol™ Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications involving polar and hydrophilic compounds. Manufactured using ultra-high purity silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent durability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for established methods and preparative method development.

82560-P21-100 is packed with 5 µm NP Diol™ particles featuring a 100 Å pore size in a 21.2 mm ID x 100 mm preparative format. The large internal diameter provides substantial sample loading capacity for purification, fraction collection, and analytical-to-preparative method scale-up. NP Diol™ is frequently selected as an alternative to unmodified silica columns because it often offers improved robustness and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 21.2 mm ID x 100 mm format is ideal for preparative HILIC and normal-phase chromatography applications involving hydrophilic and polar compounds. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition, system pressure limitations, and application requirements. A specialized preparative HPLC system capable of high flow rates and large sample loading is required for optimum performance.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity silica support

• Terminal diol functional group

• 100 Å pore size

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Alternative to unmodified silica columns

• High sample loading capacity

• Broad solvent compatibility

Typical Applications

• Preparative HILIC chromatography

• Preparative normal-phase HPLC

• Compound purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Polar compound isolation

• Hydrophilic compound purification

• Process development

• Alternative USP L20 separations

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: NP Diol™

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 21.2 mm ID x 100 mm

• Recommended Flow Rate: 15-25 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information page for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC and normal-phase preparative chromatography

• Often used as an alternative to USP L20 columns

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• 21.2 mm ID format provides substantial sample loading capacity

• 100 mm length offers an excellent balance of throughput and resolution

• Typical operating range is approximately 15-25 mL/min

• Requires a specialized preparative HPLC system capable of high flow rates and sample loading

• Ideal for purification, fraction collection, and method scale-up

• This is a Special Order Item