Catalog Number - 82560-25P-4

Cogent NP Diol™ HPLC Columns are designed for normal-phase and HILIC separations of polar and hydrophilic compounds. Manufactured using ultra-high purity silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase provides reproducible retention, excellent stability, and broad solvent compatibility. The non-endcapped surface chemistry offers selectivity consistent with USP L20 classifications, making it an excellent choice for both established methods and new method development.

82560-25P-4 is packed with 5 µm NP Diol™ particles featuring a 100 Å pore size in a 4.0 mm ID x 250 mm format. This highly efficient column configuration provides maximum resolving power and peak capacity for complex separations while maintaining the robustness expected from the NP Diol™ stationary phase. NP Diol™ columns are frequently selected as alternatives to unmodified silica columns because they often provide greater durability and compatibility with a wider range of solvents while maintaining similar retention characteristics for many applications.

The 4.0 mm ID x 250 mm format is ideal for demanding HILIC applications, normal-phase chromatography, pharmaceutical analysis, quality control testing, and method development requiring maximum resolution. Typical flow rates are approximately 0.8-1.2 mL/min depending on mobile phase composition and application requirements.

Key Benefits

• NP Diol™ stationary phase

• USP L20 equivalent chemistry

• Ultra-high purity 100 Å silica support

• Terminal diol functional group

• Suitable for HILIC applications

• Suitable for normal-phase chromatography

• Alternative to unmodified silica columns

• Broad solvent compatibility

• Maximum resolution and peak capacity

• Popular choice for challenging separations

Typical Applications

• HILIC chromatography

• Normal-phase HPLC

• Polar compound analysis

• Hydrophilic compound analysis

• Pharmaceutical analysis

• Method development

• Quality control testing

• Carbohydrate analysis

• Metabolite analysis

• Routine analytical HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: NP Diol™

• Chromatography Mode: HILIC / Normal Phase

• Functional Group: Diol

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 4.0 mm ID x 250 mm

• Recommended Flow Rate: 0.8-1.2 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Suitable for HILIC and normal-phase chromatography

• Often used as an alternative to USP L20 columns

• Provides retention of hydrophilic and polar compounds

• More robust than many unmodified silica columns

• Broad solvent compatibility for method flexibility

• 250 mm length provides maximum resolution and peak capacity

• Ideal for complex separations and method development

• Useful for carbohydrates, metabolites, and other polar analytes

• Suitable for both established methods and new method development

• 4.0 mm ID format is compatible with most standard HPLC systems

• This is a Special Order Item