HPLC Column, NP Silica, Wide Pore, 3um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent NP Silica™ Wide Pore HPLC Columns are designed for the separation of large hydrophilic and polar compounds using Normal Phase and HILIC chromatography modes. Manufactured from ultra-high purity, low metal content 300Å silica that is not chemically modified, this stationary phase provides the native silica selectivity required for challenging separations involving larger molecules. NP Silica™ Wide Pore is widely used as an alternative for USP L3 methods involving biomolecules and other high molecular weight compounds, as well as for new analytical method development.
82361-05P-4-3M is packed with 3 µm NP Silica™ Wide Pore particles in a 4.0 mm ID x 50 mm analytical format. The short 50 mm length is optimized for rapid analysis, method development, and high-throughput screening applications. The 3 µm particle size delivers higher chromatographic efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating backpressure should be expected. The 4.0 mm internal diameter provides a specialized analytical format for methods requiring this specific column geometry.
NP Silica™ Wide Pore is commonly used for large hydrophilic and polar compounds in both HILIC and Normal Phase chromatography. Because the stationary phase is completely unmodified, it provides the classic selectivity associated with bare silica while the 300Å pore structure improves accessibility for larger analytes. This format is particularly useful for proteins, peptides, biomolecules, and other compounds that may experience restricted pore access with conventional pore-size silica materials.
Key Benefits
NP Silica™ Wide Pore stationary phase
USP L3 equivalent chemistry
Ultra-high purity silica
Low metal content silica
Unmodified silica surface
300Å wide pore design
3 µm particle size
Higher efficiency than 5 µm particles
Suitable for HILIC separations
Suitable for Normal Phase chromatography
Typical Applications
USP L3 methods
HILIC chromatography
Normal Phase HPLC
Large molecule analysis
Protein analysis
Peptide analysis
Biomolecule separations
Polar compound analysis
Method development
Established silica-based methods
Specifications
Product Type: Analytical HPLC Column
Stationary Phase: NP Silica™ Wide Pore
USP Classification: L3
Chromatography Modes: HILIC and Normal Phase
Particle Size: 3 µm
Pore Size: 300Å
Column Dimensions: 4.0 mm ID x 50 mm
Recommended Flow Rate: 0.8–1.8 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Suitable for HILIC and Normal Phase chromatography
USP L3 equivalent chemistry
Unmodified silica stationary phase
Designed for large hydrophilic and polar compounds
Low metal content, high-purity silica
Wide pore 300Å silica provides improved accessibility for larger molecules
Provides higher efficiency than comparable 5 µm columns
Generates higher operating backpressure than 5 µm particles
Compatible with analytical HPLC systems capable of supporting 3 µm particle columns
Frequently used for large molecule and established silica-based methods
50 mm length minimizes solvent consumption and analysis time
This is a Special Order Item