HPLC Column, NP Diol, Wide Pore, 3um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent NP Diol™ Wide Pore HPLC Columns are designed for the separation of hydrophilic, polar, and larger molecular weight compounds using Normal Phase and HILIC chromatography modes. Manufactured using high-purity 300Å wide-pore silica bonded with a short hydrocarbon ligand containing a terminal diol functional group, this stationary phase is not end capped and provides enhanced accessibility for larger molecules while maintaining the selectivity expected from a diol phase. NP Diol™ Wide Pore columns are widely used as an alternative for USP L20 methods and for analytical methods involving biomolecules, proteins, peptides, and other large molecular weight analytes.
82360-05P-4-3M is packed with 3 µm NP Diol™ Wide Pore particles in a 4.0 mm ID x 50 mm analytical format. The short 50 mm length is optimized for rapid analysis, method development, and high-throughput screening applications. The 3 µm particle size provides higher efficiency and sharper peak shapes than comparable 5 µm columns, although increased operating pressure should be expected. The 4.0 mm internal diameter provides an alternative analytical format for methods that require this specific column geometry.
The NP Diol™ Wide Pore stationary phase is commonly selected for hydrophilic and polar compounds analyzed by HILIC and Normal Phase chromatography, particularly when larger molecules require greater pore accessibility. It is frequently used as a replacement for unmodified silica columns because it offers improved robustness and broader solvent compatibility while maintaining strong polar selectivity. The 300Å pore structure is especially beneficial for larger analytes that may experience restricted access in standard pore-size materials.
Key Benefits
NP Diol™ Wide Pore stationary phase
USP L20 equivalent chemistry
High-purity 300Å silica
Diol bonded phase
Wide pore design for large molecules
3 µm particle size
Higher efficiency than 5 µm particles
Sharper peak shapes
Suitable for HILIC separations
Suitable for Normal Phase chromatography
Typical Applications
USP L20 methods
HILIC chromatography
Normal Phase HPLC
Large molecule analysis
Protein analysis
Peptide analysis
Biomolecule separations
Polar compound analysis
Method development
Replacement of silica columns
Specifications
Product Type: Analytical HPLC Column
Stationary Phase: NP Diol™ Wide Pore
USP Classification: L20
Chromatography Modes: HILIC and Normal Phase
Particle Size: 3 µm
Pore Size: 300Å
Column Dimensions: 4.0 mm ID x 50 mm
Recommended Flow Rate: 0.8–1.8 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
Please review the Special Order Item Information for complete details.
For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.
Important Notes
Suitable for HILIC and Normal Phase chromatography
USP L20 equivalent chemistry
Not end capped
Designed for hydrophilic and polar compounds
Wide pore 300Å silica provides improved accessibility for larger molecules
Provides higher efficiency than comparable 5 µm columns
Generates higher operating backpressure than 5 µm particles
Compatible with analytical HPLC systems capable of supporting 3 µm particle columns
Often selected as a more robust alternative to unmodified silica columns
Broad solvent compatibility
50 mm length minimizes solvent consumption and analysis time
This is a Special Order Item