Catalog Number - 82061-SP250

Cogent NP Silica™ Semi-Preparative HPLC Columns are trusted by laboratories worldwide for purification, method scale-up, and compound isolation applications. Manufactured from ultra-high purity, low metal content, 100Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for the separation and purification of hydrophilic and polar compounds. Suitable for both HILIC and Normal Phase chromatography, this stationary phase is equivalent to USP L3 silica chemistry and has been successfully used for many years in pharmaceutical, cannabis, chemical, and forensic laboratories.

82061-SP250 is packed with 10 µm NP Silica™ particles in a 10 mm ID x 250 mm semi-preparative column format. The larger particle size enables operation at higher flow rates while generating lower backpressure than smaller particle columns. The 10 mm internal diameter provides significantly greater loading capacity than analytical columns, while the 250 mm length maximizes retention, peak capacity, and chromatographic resolution for demanding purification workflows.

The 10 µm particle design makes this column particularly valuable for preparative method scouting, purification method development, and scale-up studies. Because the stationary phase chemistry remains consistent across analytical, semi-preparative, preparative, and process-scale formats, methods developed on analytical NP Silica™ columns can often be transferred directly to larger ID columns with predictable chromatographic behavior. This helps streamline purification workflows, reduce development time, and simplify scale-up to preparative and process columns.

Key Benefits

• NP Silica™ stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 100Å pore size

• 10 µm particle size

• Lower operating backpressure

• Increased loading capacity

• Ideal for semi-preparative purification

Typical Applications

• Semi-preparative HPLC

• Compound purification

• Fraction collection

• Polar compound separations

• Hydrophilic compound purification

• HILIC chromatography

• Normal Phase chromatography

• Pharmaceutical purification

• Method development

• Scale-up studies

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: NP Silica™

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 100Å

• Column Dimensions: 10 mm ID x 250 mm

• Recommended Flow Rate: 8–15 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• Designed for purification of hydrophilic and polar compounds

• Lower operating pressure than smaller particle columns

• Increased loading capacity compared to analytical columns

• Frequently used for purification method development and semi-preparative separations

• Excellent for preparative method scouting and scale-up studies

• Methods can often be transferred directly to larger preparative and process-scale NP Silica™ columns

• Requires a semi-preparative HPLC system capable of supporting flow rates of approximately 8–15 mL/min

• 250 mm length provides maximum retention, peak capacity, and chromatographic resolution for demanding purification applications

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

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