Catalog Number - 82061-P21-2503M

Cogent NP Silica™ Wide Pore Preparative HPLC Columns are trusted by leading laboratories and pilot plants worldwide for compound purification, method scale-up, and process chromatography applications. Manufactured from ultra-high purity, low metal content, 300Å silica that remains completely unmodified, this stationary phase provides the native silica selectivity required for the purification of proteins, peptides, biomolecules, lipids, and other large hydrophilic or polar compounds. The wide pore structure improves analyte accessibility and is especially beneficial for larger molecular weight compounds in both HILIC and Normal Phase chromatography.

82061-P21-2503M is packed with 10 µm NP Silica™ Wide Pore particles in a 21.2 mm ID x 250 mm preparative column format. The larger particle size allows operation at higher flow rates while generating substantially lower backpressure than smaller particle columns. The 21.2 mm internal diameter provides high sample loading capacity, while the 250 mm length maximizes retention, peak capacity, and chromatographic resolution for demanding preparative separations and large-scale purification workflows.

For many years, 10 µm NP Silica™ Wide Pore columns have been used in pharmaceutical, biotechnology, cannabis, chemical, and forensic laboratories around the world for preparative HPLC and purification applications. The 300Å pore structure provides improved access for proteins, peptides, and other large molecules, while the stationary phase chemistry scales directly from analytical to preparative and purification formats. Methods developed on analytical NP Silica™ Wide Pore columns can often be transferred directly to larger ID columns with predictable chromatographic behavior, making this column an excellent choice for production purification, process development, compound isolation, and pilot-scale separations.

Key Benefits

• NP Silica™ Wide Pore stationary phase

• USP L3 equivalent chemistry

• Ultra-high purity silica

• Low metal content silica

• Unmodified silica surface

• 300Å wide pore design

• 10 µm particle size

• Lower operating backpressure

• High sample loading capacity

• Proven for preparative HPLC and purification

Typical Applications

• Preparative HPLC

• Protein purification

• Peptide purification

• Biomolecule isolation

• Large molecule separations

• HILIC chromatography

• Normal Phase chromatography

• Pharmaceutical purification

• Cannabis purification

• Process-scale chromatography

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: NP Silica™ Wide Pore

• USP Classification: L3

• Chromatography Modes: HILIC and Normal Phase

• Particle Size: 10 µm

• Pore Size: 300Å

• Column Dimensions: 21.2 mm ID x 250 mm

• Recommended Flow Rate: 15–40 mL/min

• Sample Loading Capacity: Preparative Scale

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Suitable for HILIC and Normal Phase chromatography

• USP L3 equivalent stationary phase

• Unmodified silica surface chemistry

• Manufactured from ultra-high purity, low metal content silica

• 300Å wide pore structure provides improved accessibility for proteins, peptides, and other large molecules

• Designed for purification of large hydrophilic, polar, and charged compounds

• Lower operating pressure than smaller particle preparative columns

• High loading capacity for compound isolation and purification

• Frequently used in pharmaceutical, biotechnology, cannabis, chemical, and forensic laboratories worldwide

• Proven preparative HPLC technology used globally for many years

• Directly scalable from analytical NP Silica™ Wide Pore column methods

• Excellent for purification method development, process scale-up, pilot plant operations, and production chromatography

• Requires a preparative HPLC system capable of supporting flow rates of approximately 15–40 mL/min and larger column dimensions

• 250 mm length provides maximum retention, peak capacity, and chromatographic resolution for demanding purification applications

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.